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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1 ml
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文献和实验Detection of Known Allergen-Specific IgE Antibodies by Immunological Methods
are incubated with the respective diluted serum sample (approximately 25 μL serum required) and bound IgE antibodies are detected with a secondary horseradish peroxidase (HRP) labelled anti-human-IgE antibody via chemiluminescence. The measurement
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Early development of primary motor neurons and somites in Zebrafish Embryos
using a horseradish peroxidase-conjugated secondary antibody, although more time consuming, resulted in a better signal-to-noise ratio and clearer staining .
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