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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
40ug
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文献和实验and has alternative targets,e.g., inositol–phosphate phosphatases. Physiological concentrationsof zinc ions have also been shown to directly inhibit GSK-3 in vitroin an ATP-noncompetitive manner (18) . It has been shown thattreatment of HEK 293 cells with 20 μM ZnCl
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
in H20. Use immediately. 6. Rinse in 20mM EDTA in PBS for 2-4 hours. Tissue can be stored in the dark at 40 C in PBS + EDTA or 30% sucrose in PBS + EDTA + 0.05% sodium azide for many months, although the background clearly increases
Methods to Detect Protein Glutathionylation
buffer containing 20 mM NEM. For DTT‐treated samples, extracts were treated with 10 mM DTT for 10 min at room temperature. Cell extracts (20 µg) were resolved by nonreducing SDS‐PAGE, and global protein glutathionylation and GAPDH expression were analyzed
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