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- Abnova
- ABN-CS0010-100uL
- 2026年02月19日
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- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
100uL
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文献和实验Visual DNA Detection and SNP Genotyping Using Asymmetric PCR and Split DNA Enzymes
from within human genomic DNA samples. Our approach adopts a split probe targeting system, designed with G-rich sequences, which reassembles in the presence of target DNA, producing G-quadruplexes with catalytic activity. Asymmetric PCR is first performed to amplify
beacon probe as a fluorescent reporter. The sensor has a straightforward design, and demonstrates improved selectivity and specificity of nucleic acid recognition. It is cost-efficient since it utilizes the same molecular beacon probe for the analysis
Replication timing by comparative hybridization
in Eppendorf tubes and freeze the pellets at -20o C. 7. Extract the DNA ( smash-&-grab with glass beads); digest 1/4 to 1/3 of it with the enzyme of choice in 40-60 microliters (EcoRI works well, as it cuts the ARS-less circle just once). Split
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