- 询价
- OZ Biosciences
- OZB-PL00110-100ug
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
100ug
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文献和实验BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
: For most cell lines tested (e.g. HEK293, A549, HeLa), we obtain a readily detectable fluorescence signal when using 100 nM BLOCK-iT™ Fluorescent Oligo for transfection. Prepare and seed mammalian cells at a density recommended by the manufacturer
analysis of gene expression (DSAGE) measures global gene transcript levels from only 1 to 2 µg total RNA by massively parallel sequencing of cDNA tags. This unit describes the construction of 21?bp cDNA tag libraries appropriate for massively parallel
is separated from 14C Cm by phase extraction. ASSAY Make up cocktail, keep on ice; does 10 assays 0.25 M Tris-Cl pH 8.0 0.7 ml (0.07/assay) 14C Cm (57µCi/µmol; 25µCi/ml) 0.1 ml (10µl/assay) Bu CoA (5mg/ml) 0.2 ml (20µM/assay) Total 1.0 ml (100µl/assay
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