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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
1mg
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. 36. Resuspend the cells in OP9 medium, seed 5 x 105 cells per 10-cm dish containing ~80% confluent OP9 cells in 10 mL of OP9 medium, and add 5 ng/mL of recombinant human Flt-3L. Return the dish to the incubator. The number of 10-cm dishes
50T/100T 800/1200 L0031 Mouse anti-Human CD11a mAb,PE 50T/100T 1000/1500 L0032 Mouse anti-Human CD11b mAb,FITC 50T/100T 800/1200
Intracellular Cytokine Staining Protocol
)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinJES6-5H4IL-4mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinBVD6-24G2IL-6mouse
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