APC anti-mouse/human CD11b

APC anti-mouse/human CD11b Antibody
Catalog# / Size 101211 / 25 µg
101212 / 100 µg
Clone M1/70
Other Names αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype Rat IgG2b, κ
Description CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly
-40. CD11b is a member of the integrin family, primarily expressed on granulocytes,
monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b noncovalently
associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in
cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242),
iC3b, and fibrinogen.
Product Details
Reactivity Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Antibody Type Monoclonal
Host Species Rat
Immunogen C57BL/10 splenocytes
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with APC under optimal
conditions.
Concentration 0.2 mg/ml
Storage & Handling The CD11b antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric
analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in
100 µl volume. It is recommended that the reagent be titrated for optimal performance for each
application.
Excitation Laser Red Laser (633 nm)
Application Notes Clone M1/70 has been validated for immunocytochemistry (ICC) and frozen immunohistochemistry
(IHC-F).
Additional reported applications (for relevant formats of this clone) include: immuno
6. Takeda K, et al. 2014. Am J Pathol. 184:686. PubMed
7. Iwata-Yoshikawa N, et al. 2014. J Virol. 88:8597. PubMed
8. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
9. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
10. Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed
11. Eddens T, et al. 2015. J Immunol. 195: 185 - 193. PubMed
12. Kolan S, et al. 2015. PLoS One. 10: 0134113. PubMed
RRID AB_312794 (BioLegend Cat. No. 101211)
AB_312795 (BioLegend Cat. No. 101212)
Antigen Details
Structure Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
Function Adhesion, chemotaxis
Ligand/Receptor ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience,
Neuroscience Cell Markers
Molecular Family Adhesion Molecules, CD Molecules
Antigen References 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.
Gene ID 16409
3684
Related Protocols
Cell Surface Flow Cytometry Staining Protocol
Biotin anti-mouse/human CD11b, FITC anti-mouse/human CD11b, PE anti-mouse/human CD11b, PE/Cy5 anti-mouse/human CD11b,
Purified anti-mouse/human CD11b, PE/Cy7 anti-mouse/human CD11b, Alexa Fluor® 488 anti-mouse/human CD11b, Alexa Fluor® 647
anti-mouse/human CD11b, Alexa Fluor® 700 anti-mouse/human CD11b, Pacific Blue™ anti-mouse/human CD11b, APC/Cy7 antimouse/human
CD11b, PerCP/Cyanine5.5 anti-mouse/human CD11b, PerCP anti-mouse/human CD11b, Brilliant Violet 421™ antimouse/human
CD11b, Brilliant Violet 570™ anti-mouse/human CD11b, Brilliant Violet 605™ anti-mouse/human CD11b, Brilliant Violet
650™ anti-mouse/human CD11b, Brilliant Violet 711™ anti-mouse/human CD11b, Brilliant Violet 785™ anti-mouse/human CD11b,
Brilliant Violet 510™ anti-mouse/human CD11b, Ultra-LEAF™ Purified anti-mouse/human CD11b, Purified anti-mouse/human CD11b
(Maxpar® Ready), Alexa Fluor® 594 anti-mouse/human CD11b, PE/Dazzle™ 594 anti-mouse/human CD11b, APC/Fire™ 750 antimouse/human
CD11b, TotalSeq™-A0014 anti-mouse/human CD11b, Brilliant Violet 750™ anti-mouse/human CD11b, TotalSeq™-
B0014 anti-mouse/human CD11b, TotalSeq™-C0014 anti-mouse/human CD11b
Other Formats
Product Data
C57BL/6 mouse bone marrow cells were
stained with CD11b (clone M1/70) APC
(filled histogram) or rat IgG2b, κ APC
isotype control (open histogram) (gated
on total cells).
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