- 询价
- Oxford Expression Technologies
- 500312-50 ml
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
50 ml
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文献和实验Procedure for Transfection of Mammalian Cells
, remove the transfection mixture and replace with normal growth medium. Replace medium at 18-24 h following start of transfection. Assay cell extracts for gene activity 24-72 h after the start of transfection, depending on cell type
Transfection protocol for 6-well dish
Seed cells at appropriate density (5x104/well for 3T3, MPAC, BHK; 5x105/well for aTC1.6, bTC3) one to two days before transfection. Combine total DNA at 1ug /well with 600ul/well serum-free medium in an eppendorf tube. Add 1.5ul/well Transfast
CaPO3 Transfection in 293 T cells
to distribute the DNA. Incubate the cells and DNA for 8-16 hours. Gently remove the media and replace with 2 ml of complete DMEM. Metabolically label cells the following night and IP 36-48hrs after transfection.
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