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- ABIN6923142-135 uL
- 2026年02月19日
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- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
135 uL
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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
or 0.22µm filter to prepare for L5 purification. Part II. Resin Preparation 1. Thoroughly suspend the resin by gentle inversion until the bottle of Anti- DYKDDDDK-tag (L5) affinity gel is a uniform suspension of gel beads. Transfer
Purification Of Anti Peptide Antibody
a parastaltic pump and recirculate the serum ON or just batch bind the serum ON. Wash with 5 col vols TBS. Wash with 10 col vols 0.5 M NaCl, 20mM TrisCl pH 7.4, 0.2 Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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