- 询价
- Protein Ark
- HiFliQ5-NiNTA-1-1 x 5 Column
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
1 x 5 Column
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文献和实验6xHis-tagged protein purification using Qiagen Ni-NTA Column
lysate and mix gently by shaking at40℃for 60’Load the lysate-Ni-NTA mixture into a column with the bottom outlet capped (5ml per column)Remove bottom cap and collect the column flow throughWash twice with 4ml washing buffer every time for each column
6xHis-tagged protein purification using Qiagen Ni-NTA Column under Native Condit
lysate and mix gently by shaking at40℃for 60’Load the lysate-Ni-NTA mixture into a column with the bottom outlet capped (5ml per column)Remove bottom cap and collect the column flow throughWash twice with 4ml washing buffer every time for each column
in the column matrix!! 8)Wash column with 5 column volumes of lysis buffer containing no DMSO!! --> NOTE: DMSO strips proteins off of the coulumn! 9)Run a 40 ml salt gradient collecting 1 to 2 ml fractions: - Start at 0 mM NaCl and end with 500 mM NaCl. 10
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