- 询价
- LGC(Lucigen)
- 30086-1-200 rxn
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
200 rxn
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文献和实验RNA isolation protocol: cells in culture
(per sample): RQ1 Rnase free Dnase: 1 μl Dnase 10x reaction buffer 2 μl DEPC-treated H2 O 6 μl Rnase Out 0.5 μl 2. Make a master mix of the above based on the number of RNA samples being treated. 3. Prepare
200 µl of nuclease free water and extract the RNA with Phenol:Chloroform5.Ethanol precipitate your reactions6.Resuspend the pellet in 100 µl - 200 µl water.7.Heat your reactions tubes at 70?C for 30 min.8.Place tubes in a beaker of 80?C water
DNase I Treatment Of Total RNA
from QiagenAccording to Invitrogen, reactions can be set up in a minimum of 10 ul and scaled up if larger volumes of RNA need to be treated. Treatments can range from 22-37°C for 15-30 minutes using 0.1-3 units of DNase I.Invitrogen's suggested mix:1 ug RNA1 ul DNaseI
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