- 询价
- LGC(Lucigen)
- EC300110-20 rxns
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
20 rxns
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文献和实验Transformation of Electrocompetent E. coli with Blue/White selection
's bacteria suspension. Materials Control DNA (e.g. pBS) diluted to 1 pg/µL in sterile H2 O, on ice. Electrocompetent bacteria, frozen (50 - 100 µL aliquots), on ice. BioRad cuvettes (1 mm gap), on ice. Sterile L-Broth
Transformation of E. coli by Electroporation
antibiotic SOC medium 实验步骤 1. Inoculate a single colony of E. coli from a fresh agar plate into a flask containing 50 ml of LB medium. Incubate the culture overnight at 37°C with vigorous aeration (250 rpm in a rotary
Transformation of E. coli by Electroporation电穿孔转化E.coli
. 2.Add 1 mL from overnight culture to 250 mL LB flask; grow until bacteria are in log phase (4-5 hours for E. coli) 3.Split culture across two centrifuge flasks 4.Centrifuge at 2600g for 15 min in a 4 ℃ superspeed centrifuge 5.In cold room or on ice
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