- 询价
- LGC(Lucigen)
- 60502-2-24 rxns (DUOs)
- 2026年02月19日
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10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
24 rxns (DUOs)
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文献和实验Belcher/Knight: Electrocompetent Cells
Electrocompetent Cells From Danijela Dukovski at Harvard Medical School. This protocol works well. --Julie Norville Materials DI water 10% Glycerol Special Equipment Centrifuge Ice water bath Liquid
Transformation of Electrocompetent E. coli with Blue/White selection
to determine the efficiency of competent cells: Efficiency (Transformants/µg) = colonies/plate x (Bacterial dilution factor) x 20,000 Highly competent cells should have ~50 colonies on the plate with a 1/10 dilution of the control DNA
Preparation of Electrocompetent Cells and Electroporation of Plasmid DNA
Preparation of Electrocompetent Cells and Electroporation of Plasmid DNA Inoculate 5-ml L-broth with a single colony of E. coli . Incubate 5 hours to overnight at 37°C on a roller or with moderate shaking.
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