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- ImmunoChemistry Technologies
- 266-1 plate
- 2026年02月19日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
1 plate
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文献和实验Mag-Bind Plant RNA Protocol using 96-well Plate
rotor for 96 well plates 2. Sealing Film 3. 2 ml deep-well plate (Cat#EZ9602-02 ) 4. 500 μl round well processing plate (Cat#EZ9604-02 ) 5. Equipment for disrupting plant tissue (MM300 Mixer Mill or Geno/Grinder 2000)
96 Well Mating Protocol platewise
culture to a volume of LB 0.2% Rhamnose. From this recipient pool, 100ul recipient pool is aliquotted to is added to each well of Costar U-bottom 96 well plate. Then each well gets either 1-2ul of donor overnight culture. Donor dilution: Acceptable donor
96 Well Mating Protocol platewise
, 100ul recipient pool is aliquotted to is added to each well of Costar U-bottom 96 well plate. Then each well gets either 1-2ul of donor overnight culture. Donor dilution: Acceptable donor OD600 range is 0.060 to 0.300 . Measure the cultures straight
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