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10
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北京柏瑞图科技有限公司
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1 ml
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文献和实验membranes with known fluorophore concentrations (e.g., DOPC bilayers containing either BODIPY‐FL‐DHPE or Texas Red‐DHPE) Fluorescence microscope equipped with: Filter sets that match
Single and Double FISH protocols for Drosophila
on their digoxygenin and fluorescein RNA labeling spec sheets. On ice add 1 ug of linearized template DNA (3-5kb), 2ul fluorescein or digoxigenin RNA labeling mix, 2 ul 10X transcription buffer (supplied with the RNA polymerase: 0.4M Tris-HCl, pH 8.0; 60 mM MgCl2, 100
Use of Microsphere‐Supported Phospholipid Membranes for Analysis of Protein‐Lipid Interactions
1 ) Fluorophore‐labeled ligand (fluorescein maleimide‐labeled factor VIII) Binding buffer (e.g., TBS/BSA/PLV; see recipe
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