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文献和实验LINEAGE ANALYSIS USING RETROVIRAL VECTORS
together, we have been unable to count the number of cells in a clone or distinguish the morphologies of individual cells (see [26] for an example). In such cases, nuclear lacZ is very useful.If one cannot use morphological criteria to identify the types of cells carrying
[M]=A259 /15.4x103 =A259 1:2000 x0.130 adjust the final volume. Betaine monohydrate C5 H11 NO2 xH2 O; Mw=135.2g/M; (store at 4o C). Conc. Stock %(w/w) 100ml 200ml 700ml Betaine 135.2g/M 5M
Construction of BAC Libraries：Construction of a BAC library
of clonable DNA. Because of the subsequent steps required to clone DNA fragmented by shearing, most researchers fragment DNA by partial restriction enzyme digestion. The advantage of partial restriction enzyme digestions is that no further enzymatic
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