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TNF Alpha中和抗体,N
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研卉生物
大量
69002-1
NeutraKine® TNF Alpha Monoclonal antibody
TNF Alpha中和抗体,NeutraKine
小鼠
人
Neutralization, ELISA
100ug/只
Recombinant Human TNF alpha (Catalog NO. HZ-1014) inihibits the growth of L-929 cell line in the presence of actinomycin D in a dose dependent manner (blue curve). The activity elicited by recombinant Human TNF alpha (0.05 ng/mL HZ-1014) is neutralized (red curve) by Mouse anti-human TNF alpha monoclonal antibody (Catalog NO. 69002-1-Ig). The ND50 is typically 10-50ng/mL.
Recombinant human TNF alpha (Cat.NO. HZ-1014) inhibits the growth of L-929 cell line in the presence of actinomycin D in a dose dependent manner (blue curve, refer to bottom X-left Y). The activity elicited by recombinant human TNF alpha (0.05 ng/mL HZ-1014) is neutralized by mouse anti-human TNF alpha monoclonal antibody 69002-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 10-50 ng/mL. The NeutraControl mouse anti-human TNF alpha monoclonal antibody 69502-1-Ig, generated from the same immunogen, could recognize human TNF alpha, but could not neutralize human TNF alpha at the range of 0.128-10000ng/mL (green curve, refer to top X-right Y).
Indirect ELISA was carried out by coating recombinant Human TNF Alpha (Cat.NO. HZ-1014) at 70 ng/well followed by blocking and adding serial diluted TNF Alpha antibody 69002-1-Ig and 6950
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