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文献和实验Culture and Differentiation of Human Neural Stem Cells
We describe the steps in detail to expand human neural stem cells, to bank and cryopreserve the stem cells. The methods described in this protocol represent the latest improvement in tissue culture and molecular biology reagents to help
Neural Differentiation of Human Embryonic Stem Cells
Embryonic stem cells (ESCs) are pluripotent and capable of indefinite self-renewal in vitro. These features make them a highly advantageous source for deriving any cell type of the central and peripheral nervous system. We describe neural
Human Mesenchymal Stem Cell Culture for Neural Transplantation
, trauma and Parkinson’s disease. MSCs therefore provide an attractive and practical source of stem cells for reparative therapy in patients, and in this paper we describe methods for the reproducible culture and neural differentiation of human MSCs
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