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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Hyaluronidase, >500U/MG
- 供应商:
默瑞生物
- 规格:
50MG/100MG/500MG/1G/5G
| 规格: | 50MG | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 100MG | 产品价格: | 询价 |
| 规格: | 500MG | 产品价格: | 询价 |
| 规格: | 1G | 产品价格: | 询价 |
| 规格: | 5G | 产品价格: | 询价 |
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文献和实验Lacrimal Experimental Protocol (4/4/94)
modified TNE, 20 µl of Hoechst dye (1 mg/ml) and 580 µl of filtered dH2O. [For 2.5 x modified TNE, mix in a conical: 40 ml of 5 M NaCl, 1.25 ml of 1 M Tris pH 8, 0.25 ml of 0.5 M EDTA, pH to 7.4 with 5-7 drops of 1 M HCl, then make up to 50 ml with 5 M NaCl
Procedures for In Vitro Production of Bovine Embryos
- Hyaluronidase (optional) �warm up - Sterile dolphin-nose microcentrifuge tubes (and holder) - Heater (in front of microscope) - X-Plate - HEPES-TALP - Dissecting microscope - Searching instrument - TimerRemove oocytes/embryos
. Aliquots from each time point are electrophoresed on an agarose gel versus the phi-X 174 size marker (12) to determine the approximate DNA fragment size range for each sonication time point. Once optimal sonication conditions are determined, the remaining
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