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| PCR试剂 | G008 | Taq DNA Polymerase | 5000u |
| G126 | Taq DNA Polymerase | 10000U | |
| G009 | Taq DNA Polymerase | 1000U(200 μl) | |
| G013 | 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | |
| G013-dye | 2X PCR Taq MasterMix with dye | 5.0 ml (200 Rxns) | |
| G472 | Safe-Green ; 2X PCR Taq MasterMix | 5.0 ml (200 Rxns) | |
| G012 | Taq Plus DNA Polymerase | 250 U (50 µl) | |
| G040 | Taq Plus DNA Polymerase | 1000U(200 µl) | |
| G014 | 2X PCR Taq Plus MasterMix | 5.0 ml (200 Rxns) | |
| G014-dye | 2X PCR Taq Plus MasterMix with dye | 5.0 ml (200 Rxns) | |
| G277 | TaqFast DNA Polymerase | 250 U (50 µl) | |
| G278 | TaqFast DNA Polymerase | 1000U(200 μl) | |
| G280 | 2X PCR TaqFast MasterMix | ||
| G280-dye | 2X PCR TaqFast MasterMix with dye | ||
| G078 | Precision ; DNA Polymerase | 500 U (100 µl) | |
| G011 | HotStart DNA Polymerase | 250 U (50 µl) | |
| G039 | HotStart DNA Polymerase | 1000U(200 µl) | |
| G010 | dNTP Mix | 250µl | |
| G128 | dNTP Mix | 500 µl | |
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文献和实验at least 2 reference genes for subsequent reactions and normalisation. Inlcude your genorm M values when publishing qPCR data. Primer selection Main article: Choosing primers for qPCR Choosing suitable primers is an early crucial step in your qPCR experiment
. Small RNAs comprise two major RNA classes, short interfering RNAs (siRNAs) and microRNAs (miRNAs). Efficient and reliable detection and quantification of small RNA expression has become an essential step in understanding their roles in specific cells
Real-Time qRT-PCR standard curves... efficiency is too h
Greetings, In my Real-Time qRT-PCR experiments, I employ the standard curve method for quantification of gene expression. However, standard curves seem to be a huge hit-or-miss procedure for me, even with genes that are well-established
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