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- 文献和实验
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- 保存条件：
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- 英文名：
PVDF Transfer Membrane (10cm x 15cm)
- 库存：
50
- 供应商：
囊萤科技
- 规格：
1*10ea
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文献和实验Membrane Transfer and Crosslinking for RNA
The composition of the transfer buffer is usually a 5X SSC/10 mM NaOH solution. These mildly alkaline conditions shear the RNA into smaller fragments and denature it as it is deposited onto the membrane. A brief protocol for assembly (see Figure 1
Southern Transfer to Nylon Membrane in NaOH
Principle: Refer to Southern Transfer with Zetabind membrane. The NaOH transfer may be a more efficient transfer method for larger sized fragments. It is the method of choice for pulsed-field gels containing fragments >= 20 kb
Protein detection onto PVDF membranes
2-D PAGE and electroblotting onto PVDF membranes have become widely used techniques for the characterization of proteins. Recent improvements have allowed a higher protein load, a better transfer yield and a higher sensitivity in automated
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