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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human monocytes
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性:
Monoclonal
- 标记物:
PE
- 适应物种:
Human, Pig
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX75219
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
FACS
- 浓度:
0.1 mg/ml
- 靶点:
ICAM1 / CD54
- 抗体英文名:
ICAM1 / CD54 antibody [15.2] (PE)
- 抗体名:
ICAM1 / CD54 抗体 [15.2] (PE)
- 规格:
100 test
FACS analysis of human peripheral blood monocytes using GTX75219 ICAM1 / CD54 antibody [15.2] (PE).
Solid line : Primary antibody
Dashed line : PE mouse IgG1 isotype control
Antibody amount : 0.5 μg
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文献和实验PMID: 10745021, 2000
PMID: 19429776, 2009
PMID: 21183689, 2011
PMID: 22337883, 2012
PMID: 22619321, 2012
PMID: 22287712, 2012
Eun-Ji Lim et al., Cell Death Dis 2022 (PMID:35487888)
Chiang HY et al., J Biomed Sci 2019 (PMID:31470852)
Lin JC et al., Neoplasia 2019 (PMID:30959276)
Fan JR et al., Cell Death Dis 2018 (PMID:29717132)
Lee TC et al., PLoS One 2014 (PMID:25380245)
Tran DQ et al., J Immunol 2009 (PMID:19234188)
PMID: 15943783, 2005
PMID: 9151884, 1997
PMID: 20977750, 2010
Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
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