- ￥3200
- GeneTex
- 美国
- GTX01470-08
- 2026年02月13日
- FACS
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
- 克隆性：
Monoclonal
- 标记物：
PE
- 适应物种：
Mouse
- 保质期：
12 months from the shipping date of the product.
- 目录编号：
GTX01470-08
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rat
- 应用范围：
FACS
- 浓度：
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
F4/80
- 抗体英文名：
F4/80 antibody [BM8.1] (PE)
- 抗体名：
F4/80 抗体 [BM8.1] (PE)
- 规格：
100 μg
FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01470-08 F4/80 antibody [BM8.1] (PE).
Right panel : co-stained with F4/80 antibody [BM8.1] (PE) and Mouse CD11b antibody (APC)
Left panel : co-stained with isotype control and Mouse CD11b antibody (APC)
antibody amount : 0.25 μg (5 μl)
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文献和实验PMID: 23269244, 2013
PMID: 23264656, 2013
PMID: 23382560, 2013
PMID: 16037409, 2005
PMID: 20042577, 2010
Western Blotting with Horseradish Pe
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
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