- ￥5000
- GeneTex
- 美国
- GTX60332
- 2026年02月18日
- ELISA, ChIP assay
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related)) using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60332
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
ELISA, ChIP assay
- 靶点：
ETO / RUNX1T1
- 抗体英文名：
ETO / RUNX1T1 antibody - ChIP grade
- 抗体名：
ETO / RUNX1T1 抗体 - ChIP grade
- 规格：
50 μl
ChIP analysis of sheared chromatin from 1.25x10⁶ SKNO-1 cells using GTX60332 ETO / RUNX1T1 antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. This figure shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used.
Antibody amount : 4μl
ChIP analysis of SKNO-1 cells using GTX60332 ETO / RUNX1T1 antibody - ChIP grade. The IP'd DNA of 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
ELISA analysis of peptides used for immunization using GTX60332 ETO / RUNX1T1 antibody - ChIP grade.
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文献和实验Reagents: ChIP sonication Buffer (1% Triton X-100,0.1% Deoxycholate,50 mM Tris 8.1,150 mM NaCl,5 mM EDTA):10 ml 10% Triton X-1001 ml 10% Deoxycholate5 ml 1 M Tris-Cl pH 8.11 ml 0.5 M EDTA3 ml 5 M NaCl80 ml WaterJust before use,add 10μl Aprotinin,10μ
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
) Antibody directed against protein of interest (e.g., Upstate Biotechnology, Santa Cruz Biotech, Abcam) Triton X‐100
RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells
Back to top Figures Figure 1. An example of an RNA-ChIP experiment to examine interactions between the DNA methyltransferase, Dnmt3a, and the noncoding RNA, Tsix (see Reference 1). In wild-type male (X/Y) and female (X/X) cells, Tsix RNA can be amplified
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