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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CDC2. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX108120
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
IP/MS validation was supported by references (PMID:30377391)
- 抗体英文名:
CDC2 antibody [N1], N-term
- 抗体名:
CDC2 抗体 [N1], N-term
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX108120 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min.
Green: CDC2 protein stained by CDC2 antibody [N1], N-term (GTX108120) diluted at 1:750.
Blue: Hoechst 33342 staining.
Sample (30 μg of whole cell lysate)
A: PC-12
10% SDS PAGE
GTX108120 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CDC2 antibody [N1], N-term (GTX108120) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: A549
B: H1299
C: HCT116
10% SDS PAGE
GTX108120 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC2 antibody [N1], N-term (GTX108120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Ko PH et al., Sci Rep 2019 (PMID:30765775)
Ming-YangLee et al., European Journal of Pharmacology 2018 839()
Sikorski K et al., Nat Methods 2018 (PMID:30377371)
Lu WJ et al., Food Funct 2018 (PMID:30083664)
Li TN et al., J Pathol 2018 (PMID:29862509)
Huang TH et al., Mol Cell 2018 (PMID:29478807)
Chin YT et al., Oncotarget 2014 (PMID:25436977)
Yuan SY et al., Oncol Rep 2017 (PMID:28075477)
Cheung AK et al., Mol Cancer Ther 2013 (PMID:23686835)
Chu CS et al., J Biol Chem 2011 (PMID:21852232)
Pan J et al., Oncogene 2010 (PMID:20802531)
Xiaobo Wang et al., J Radiat Res 2022 (PMID:35333349)
Yanqi Xie et al., Mol Cancer Ther 2021 (PMID:34376582)
Yongfeng Zhou et al., FEBS Open Bio 2022 (PMID:34856073)
Kuo TT et al., ACS Omega 2020 (PMID:33344833)
Lin JH et al., Stem Cells International 2019 2019()
Chen WT et al., Int J Oncol 2019 (PMID:31322205)
Chueh-Hsuan Lu et al., bioRxiv 2019 (Epub)
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
in pEGFP-N1 is between the immediate early promoter of CMV (PCMV IE ) and the EGFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening
to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain
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