- ￥3737
- Merck
- 美国
- 598502-100UG
- 2026年02月13日
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文献和实验, resuspend in 2 ml 1 x LiAc/ 0.5 x TE incubate at RT for 10 min combine in tube: 100 µl cells, 10 µl salmon sperm DNA (10 mg/ml), up to 15 µl DNA to be transformed add 700 µl 1 x LiAc / 1 x TE / 40 % PEG mix incubate for 30 min
. and resuspend in 1.5mL TE/LiAC. Add 100ul cells to the plasmid DNAs (1-5 ug) and single-stranded carrier DNA (20ug) in a microfuge tube. The carrier DNA is produced by dissolving salmon sperm DNA in TE, sonicating it to reduce its viscosity, extracting
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST
with 0.5 mM PMSF. Spin down @ 5K, 5 minutes, 4℃. Drain. Gently resuspend cell pellet in 5 mL cold Triton X/HEPES with 0.5 mM PMSF, 0.8 ug/mL pepstatin A, and 0.6 ug/mL leupeptin. Spin down @ 7 K, 7 minutes, 4℃. Drain. Gently resuspend cell pellet
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