a-JNK3/SAPK1b, clone C05T

Chromosome Conformation Capture (3C) (PROT05)

C and add desired amount of T4 DNA ligase. Incubate for 4 hours (see note 4 ). DNA Purification Cross-links are reversed and the DNA is purified prior to PCR analysis. Add proteinase K (100µg/ml final) and incubate at 65°C overnight

Single Clone Excision Protocol

500ml 20% maltose and 500ml 1 M MgSO4 to 50 ml LB broth). Next day: 3.If you're making new cells,spin them down 5 min at 1500 x g in the C0650 rotor in the Beckman centrifuge,then resuspend in about 40 ml 10 mM MgSO4,and quantify absorbance

Clone Genes From a Phage Library

from the desired phage Some preparatory items The library (one that I made from C. albicans genomic DNA) is kept at -80 degrees C in 0.5 ml aliquots. This solution is also stable in the refrigerator for several months at least. The library