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文献和实验DNA Origami: Synthesis and Self‐Assembly
origami assemblies using a four‐way connector. Chem. Commun. 47:3213‐3215. Endo, M., Hidaka, K., and Sugiyama, H. 2011b. Direct AFM observation of an opening event of a DNA
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
Transformation of Competent Cells
no more than a few hours before use. Alternately, aliquot the cell suspension into 1.7-ml microcentrifuge tubes. Flash freeze by placing tubes in a dry ice/ethanol bath until frozen, and store at -70°C. B
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