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文献和实验Purifying the Hedgehog Protein and its Variants
The purification of recombinant versions of the N-terminal signaling fragment of Sonic hedgehog (ShhN) from E. coli , Hi-5™ insect cells, yeast, and mammalian cell sources reveals diverse post-translational modifications that affect
Identification of Human Pancreatic Cancer Stem Cells
also demonstrate upregulation of molecules important in developmental signaling pathways, including sonic hedgehog and the polycomb gene family member Bmi-1. Of clinical importance, cancer stem cells in several tumor types have shown resistance to standard
Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells
from the adult human bone marrow (BM) can be easily isolated and expanded in culture while maintaining their immense plasticity. Here, we describe a protocol to generate DA-producing cells from adult human MSCs using a cocktail that includes sonic hedgehog (SHH
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