- ¥1310 - 19710
- 江蓝纯
- P00068
- 2025年07月12日
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- 库存：
99
- 供应商：
江蓝纯
- 规格：
10ug/50ug/500ug/1mg
| 规格： | 10ug | 产品价格： | ¥1310.0 |
|---|---|---|---|
| 规格： | 50ug | 产品价格： | ¥3920.0 |
| 规格： | 500ug | 产品价格： | ¥13770.0 |
| 规格： | 1mg | 产品价格： | ¥19710.0 |
| Known as | Sonic Hedgehog Protein; SHH; HHG-1 |
|---|---|
| Derived from | E.coli |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. |
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months |
| Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxin | Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Background | Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc. |
Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197 is expressed.
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文献和实验人Sonic Hedgehog(SHH)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 Sonic Hedgehog ( SHH )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 SHH 单抗包被于酶标板上，标准品和样品中的 SHH与单抗结合，加入生物
Application of Sonic Hedgehog to the Developing Chick Limb
Here, we describe methods for applying Sonic hedgehog (Shh) to developing chick limbs. The Sonic hedgehog gene is expressed in the polarizing region, a signaling region at the posterior margin of the limb bud and application of Shh-expressing
SYendocrine 想请问各位大虾，sonic hedgehog通路的特异性激活剂是什么？ （附加：另外有没有哪位知道该通路在肾小球系膜细胞中是否表达，如果想测是否表达通过RT-PCR测SHH，通过Elisa法测shh蛋白就行了么？） 请各位大虾不吝赐教！！ 大鹏鸟 好像只有SHH能激活，没看到有文献说有其他激活剂的。 想测表达与否还是通过传统的方法,RT-PCR ,WESTERN，ELISA
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