- ￥1103
- Merck
- 美国
- 419800-5MG
- 2026年02月12日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
, T12 MG, T12 MT ; where M is A,C or G) dilute DNA-free RNA to 0.1 µg/µl with H2 ODEPC , keep on ice set up cDNA synthesis reaction for each degenerate anchored oligo(dT) primer set: 4 µl 5 x reverse transcriptase buffer, 2 µl DTT
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
reaction buffer can be stored for at least one year at room temperature in foil-covered container. X-gal stock(40X) 40 mg/ml X-gal in dimethylformamide Store at -200 C in foil covered glass container. Rinse many times in PBS
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