20S PROT ASSAY KIT- 100 ASSY

ChIP assay: Chromatin immunoprecipitation assay was performed according to the protocol of ChIP assay kit

  1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands

Chromosome Conformation Capture (3C) (PROT05)

  Introduction An alternative protocol (PROT31) for 3C has been adopted by the author's lab. Much of it is identical to this version. The major differences are in the amounts of DNA used at different steps. We reliably