- ￥4622
- Merck
- 美国
- MATRNPS50
- 2026年02月12日
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文献和实验Mag-Bind Plant RNA Protocol using 96-well Plate
4. Incubate 10 minutes at -20°C. This step helps to remove the proteins, polysacchrides and other inhibitors. 5. Centrifuge at 4,000 x g for 20 min to pellet cell debris. Compact pellets will form in the plate but some particles may float. Be careful
, as before, and placing the microtiter plate back on the Biomek, a Triton X-100 detergent solution is robotically added to each well. The plate is agitated gently and the sample from each pair of wells is robotically transferred to microcentrifuge tubes
Fast and reliable mini-prep RNA extraction from Neurospora crassa
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extract more than 50 minipreps at once. Procedures of RNA extraction published so far follow
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