TOLUENE 4-SULFONIC ACID SILVER

Use of Immunogold with Silver Enhancement

against a dark background (Fig. 1B ). The silver enhancement method has its basis in 19th-century photographic techniques. The enhancing solutions are physical developers that contain both silver ions and a reducing agent, buffered to an acid pH. The developers

2D Gel Electrophoresis

to remove.Polysaccharides cause severe smearing that is evident with both silver and Coomassie staining. we don't have much experience with this problem though.(e) SaltsHigh concentrations of salt can pose problems to the integrity of the IEF gel

2D Gel Electrophoresis（图）

, especially when focusing in the very basic or acid pH region.The higher the pH dependence of the mobility, (dµ /dpH), the better the focusing. A high electrophoretic mobility close to the IpH will efficiently transfer diffused protein molecule back to IpH