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文献和实验with aberrant promoter methylation.3 For example, clinical trials have shown that 5-aza-2′-deoxycytidine, a nucleoside inhibitor of DNA methyltransferase, has antitumour activity in myeloid malignant disorders.4 However, there are concerns that epigenetic
correct reconstruction of A53T and A30P.(8)结果:DNA测序结果证明构建载体插入正确α-突触核蛋白突变体A53T与A30P基因。(9)METHODS: RKO cells were treated with selective DNA methyltransferase (DNMTs) inhibitor, 5-Aza-2′-deoxycytidine (5-Aza-CdR), for 72 h. Methylation-specific PCR (MSP), T
5′-Azacytidine Expression Arrays
Epigenetic silencing of a gene can be reversed, resulting in reactivation of expression, by drugs such as the DNA methylation inhibitor 5-Aza-2′-deoxycytidine (5Aza-dC, azacytidine). This drug is added to cell culture media and is incorporated
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