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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Thyroid Peroxidase Monoclonal Antibody (6H7)
- 靶点:
Thyroid Peroxidase
- 浓度:
1 mg/ml
- 应用范围:
ELISA, Western Blot
- 宿主:
Mouse
- 适应物种:
Human
- 克隆性:
Monoclonal
- 保存条件:
4°C or -20°C if preferred
- 形态:
Liquid
- 亚型:
IgG1
- 免疫原:
Human thyroid peroxidase.
- 规格:
200 µg
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
Western Blotting with Monoclonal Ant
in all steps using HRPO (horseradish peroxidase) conjugates. 1.Decant the primary antibody solution, add wash buffer (10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20), and wash for 30 minutes with agitation, changing the wash buffer every 3 minutes. 2.Dilute
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