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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Goat F(ab')2 Anti-Mouse IgG(H+L), Human ads-APC
- CAS号：
Goat F(ab')2 Anti-Mouse IgG(H+L), Human ads-APC
- 保质期：
1年
- 供应商：
齐源生物
- 保存条件：
2-8℃
- 规格：
0.25mg
山羊F（ab'）2抗鼠Ig G（H+L），人ads-PE、Goat F(ab')2 Anti-Mouse IgG(H+L), Human ads-APC详细如下：
山羊 F(ab') 2抗小鼠 IgG(H+L)-PE 抗体，对人蛋白的反应性极低，可用于流式细胞术检测。
同型：山羊 F(ab') 2 IgG
同型对照：山羊 F(ab')2 IgG-PE
特异性：与小鼠 IgG 1、IgG 2a、IgG 2b、IgG 2c和 IgG 3的重链和轻链以及小鼠 IgM 和 IgA 的轻链 反应
来源：Goat Anti-Mouse IgG(H+L) 的胃蛋白酶消化物，人类ads（SB Cat. No. 1031）
交叉吸附：人免疫球蛋白和混合血清；可能与其他物种的免疫球蛋白发生反应
共轭：PE（R-藻红蛋白）
缓冲液配方：含有 < 0.1% 叠氮hua钠和稳定剂的磷酸盐缓冲盐水
克隆性：多克隆
浓度：0.25 毫克/毫升
体积：1.0 毫升
推荐存储：2-8℃；避免暴露在光线下；不要冻结
应用
相关格式的质量测试应用包括 -
ELISA 1,2,24,25
FLISA
流式细胞术4-20
相关格式的其他参考应用包括 -
ELISpot 3
免疫组织化学-冷冻切片21,26,27
免疫组织化学-石蜡切片21
免疫细胞化学13,17 ,22
蛋白质印迹23,28
Citations
1. Mansfield LS, Bell JA, Wilson DL, Murphy AJ, Elsheikha HM, Rathinam VA, et al. C57BL/6 and congenic interleukin-10-deficient mice can serve as models of Campylobacter jejuni colonization and enteritis. Infect Immun. 2007;75:1099-115. (ELISA)
2. Zhang H, Jia C, Xi H, Li S, Yang L, Wang Y. Specific inhibition of Candida albicans growth in vitro by antibodies from experimental Candida keratitis mice. Exp Eye Res. 2011;93:50-8. (ELISA)
3. Shaw CA, Galarneau J, Bowenkamp KE, Swanson KA, Palmer GA, Palladino G, et al. The role of non-viral antigens in the cotton rat model of respiratory syncytial virus vaccine-enhanced disease. Vaccine. 2013;31:306-12. (ELISPOT)
4. Xia Y, Ross GD. Generation of recombinant fragments of CD11b expressing the functional β-glucan-binding lectin site of CR3 (CD11b/CD18). J Immunol. 1999;162:7285-93. (FC)
5. Force WR, Glass AA, Benedict CA, Cheung TC, Lama J, Ware CF. Discrete signaling regions in the lymphotoxin-β receptor for tumor necrosis factor receptor-associated factor binding, subcellular localization, and activation of cell death and NF-κB pathways. J Biol Chem. 2000;275:11121-9. (FC)
6. Céfai D, Favre L, Wattendorf E, Marti A, Jaggi R, Gimmi CD. Role of Fas ligand expression in promoting escape from immune rejection in a spontaneous tumor model. Int J Cancer. 2001;91:529-37. (FC)
7. Samten B, Wizel B, Shams H, Weis SE, Klucar P, Wu S, et al. CD40 ligand trimer enhances the response of CD8+ T cells to Mycobacterium tuberculosis. J Immunol. 2003;170:3180-6. (FC)
8. Ritelli M, Amadori M, Tagliabue S, Pacciarini ML. Use of a macrophage cell line for rapid detection of Mycobacterium bovis in diagnostic samples. Vet Microbiol. 2003;94:105-20. (FC)
9. McNees AL, Mahr JA, Ornelles D, Gooding LR. Postinternalization inhibition of adenovirus gene expression and infectious virus production in human T-cell lines. J Virol. 2004;78:6955-66. (FC)
10. Mongini PK, Inman JK, Han H, Kalled SL, Fattah RJ, McCormick S. Innate immunity and human B cell clonal expansion: effects on the recirculating B2 subpopulation. J Immunol. 2005;175:6143-54. (FC)
11. Kawabata A, Momoi Y, Inoue-Murayama M, Iwasaki T. Canine mdr1 gene mutation in Japan. J Vet Med Sci. 2005;67:1103-7. (FC)
12. Clancy RM, Neufing PJ, Zheng P, O'Mahony M, Nimmerjahn F, Gordon TP, et al. Impaired clearance of apoptotic cardiocytes is linked to anti-SSA/Ro and -SSB/La antibodies in the pathogenesis of congenital heart block. J Clin Invest. 2006;116:2413-22. (FC)
13. Shao DD, Suresh R, Vakil V, Gomer RH, Pilling D. Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation. J Leukoc Biol. 2008;83:1323-33. (FC, ICC)
14. Garnett CT, Talekar G, Mahr JA, Huang W, Zhang Y, Ornelles DA, et al. Latent species C adenoviruses in human tonsil tissues. J Virol. 2009;83:2417-28. (FC)
15. Drewniak A, van Raam BJ, Geissler J, Tool AT, Mook OR, van den Berg TK, et al. Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes. Blood. 2009;113:5979-98. (FC)
16. Miller LA, Gerriets JE, Tyler NK, Abel K, Schelegle ES, Plopper CG, et al. Ozone and allergen exposure during postnatal development alters the frequency and airway distribution of CD25+ cells in infant rhesus monkeys. Toxicol Appl Pharmacol. 2009;236:39-48. (FC)
17. Montalvão F, Almeida GM, Silva EM, Borges VM, Vasconcellos R, Takiya CM, et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-α secretion and reduce parasite replication in macrophages. Eur J Immunol. 2010;40:417-25. (FC, ICC)
18. Chmielewski M, Hombach AA, Abken H. CD28 cosignalling does not affect the activation threshold in a chimeric antigen receptor-redirected T-cell attack. Gene Ther. 2011;18:62-72. (FC)
19. Fournier P, Aigner M, Schirrmacher V. Targeting of IL-2 and GM-CSF immunocytokines to a tumor vaccine leads to increased anti-tumor activity. Int J Oncol. 2011;38:1719-29. (FC)
20. Guzman R, Valente EG, Pretorius J, Pacheco E, Qi M, Bennett BD, et al. Expression of ORAI1, a plasma membrane resident subunit of the CRAC channel, in rodent and non-rodent species. J Histochem Cytochem. 2014;62:864-78. (FC)
21. Dasso JF, Obiakor H, Bach H, Anderson AO, Mage RG. A morphological and immunohistological study of the human and rabbit appendix for comparison with the avian bursa. Dev Comp Immunol. 2000;24:797-814. (IHC-FS, IHC-PS)
22. Crawford JR, Pilling D, Gomer RH. FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation. J Leukoc Biol. 2012;92:699-711. (ICC)
23. Tosi MF, Zakem H. Surface expression of Fcγ receptor III (CD16) on chemoattractant-stimulated neutrophils is determined by both surface shedding and translocation from intracellular storage compartments. J Clin Invest. 1992;90:462-70. (WB)
24. Rifkin IR, Leadbetter EA, Beaudette BC, Kiani C, Monestier M, Shlomchik MJ, et al. Immune complexes present in the sera of autoimmune mice activate rheumatoid factor B cells. J Immunol. 2000;165:1626-33. (ELISA)
25. Singh N, Johnstone DB, Martin KA, Tempera I, Kaplan MJ, Denny MF. Alterations in nuclear structure promote lupus autoimmunity in a mouse model. Dis Model Mech. 2016;9:885-97. (ELISA)
26. Hande S, Notidis E, Manser T. Bcl-2 obstructs negative selection of autoreactive, hypermutated antibody V regions during memory B cell development. Immunity. 1998;8:189-98. (IHC-FS)
27. Notidis E, Hande S, Manser T. Enforced expression of Bcl-2 selectively perturbs negative selection of dual reactive antibodies. Dev Immunol. 2001;8:223-34. (IHC-FS)
28. Szijártó V, Guachalla LM, Visram ZC, Hartl K, Varga C, Mirkina I, et al. Bactericidal monoclonal antibodies specific to the lipopolysaccharide O antigen from multidrug-resistant Escherichia coli clone ST131-O25b:H4 elicit protection in mice. Antimicrob Agents Chemother. 2015;59:3109-16. (WB)
29. Ohnaga T, Shimada Y, Takata K, Obata T, Okumura T, Nagata T, et al. Capture of esophageal and breast cancer cells with polymeric microfluidic devices for CTC isolation. Mol Clin Oncol. 2016;4:599-602.
山羊 F(ab') 2抗小鼠 IgG(H+L)-PE 抗体，对人蛋白的反应性极低，可用于流式细胞术检测。
同型：山羊 F(ab') 2 IgG
同型对照：山羊 F(ab')2 IgG-PE
特异性：与小鼠 IgG 1、IgG 2a、IgG 2b、IgG 2c和 IgG 3的重链和轻链以及小鼠 IgM 和 IgA 的轻链 反应
来源：Goat Anti-Mouse IgG(H+L) 的胃蛋白酶消化物，人类ads（SB Cat. No. 1031）
交叉吸附：人免疫球蛋白和混合血清；可能与其他物种的免疫球蛋白发生反应
共轭：PE（R-藻红蛋白）
缓冲液配方：含有 < 0.1% 叠氮hua钠和稳定剂的磷酸盐缓冲盐水
克隆性：多克隆
浓度：0.25 毫克/毫升
体积：1.0 毫升
推荐存储：2-8℃；避免暴露在光线下；不要冻结
应用
相关格式的质量测试应用包括 -
ELISA 1,2,24,25
FLISA
流式细胞术4-20
相关格式的其他参考应用包括 -
ELISpot 3
免疫组织化学-冷冻切片21,26,27
免疫组织化学-石蜡切片21
免疫细胞化学13,17 ,22
蛋白质印迹23,28
Citations
1. Mansfield LS, Bell JA, Wilson DL, Murphy AJ, Elsheikha HM, Rathinam VA, et al. C57BL/6 and congenic interleukin-10-deficient mice can serve as models of Campylobacter jejuni colonization and enteritis. Infect Immun. 2007;75:1099-115. (ELISA)
2. Zhang H, Jia C, Xi H, Li S, Yang L, Wang Y. Specific inhibition of Candida albicans growth in vitro by antibodies from experimental Candida keratitis mice. Exp Eye Res. 2011;93:50-8. (ELISA)
3. Shaw CA, Galarneau J, Bowenkamp KE, Swanson KA, Palmer GA, Palladino G, et al. The role of non-viral antigens in the cotton rat model of respiratory syncytial virus vaccine-enhanced disease. Vaccine. 2013;31:306-12. (ELISPOT)
4. Xia Y, Ross GD. Generation of recombinant fragments of CD11b expressing the functional β-glucan-binding lectin site of CR3 (CD11b/CD18). J Immunol. 1999;162:7285-93. (FC)
5. Force WR, Glass AA, Benedict CA, Cheung TC, Lama J, Ware CF. Discrete signaling regions in the lymphotoxin-β receptor for tumor necrosis factor receptor-associated factor binding, subcellular localization, and activation of cell death and NF-κB pathways. J Biol Chem. 2000;275:11121-9. (FC)
6. Céfai D, Favre L, Wattendorf E, Marti A, Jaggi R, Gimmi CD. Role of Fas ligand expression in promoting escape from immune rejection in a spontaneous tumor model. Int J Cancer. 2001;91:529-37. (FC)
7. Samten B, Wizel B, Shams H, Weis SE, Klucar P, Wu S, et al. CD40 ligand trimer enhances the response of CD8+ T cells to Mycobacterium tuberculosis. J Immunol. 2003;170:3180-6. (FC)
8. Ritelli M, Amadori M, Tagliabue S, Pacciarini ML. Use of a macrophage cell line for rapid detection of Mycobacterium bovis in diagnostic samples. Vet Microbiol. 2003;94:105-20. (FC)
9. McNees AL, Mahr JA, Ornelles D, Gooding LR. Postinternalization inhibition of adenovirus gene expression and infectious virus production in human T-cell lines. J Virol. 2004;78:6955-66. (FC)
10. Mongini PK, Inman JK, Han H, Kalled SL, Fattah RJ, McCormick S. Innate immunity and human B cell clonal expansion: effects on the recirculating B2 subpopulation. J Immunol. 2005;175:6143-54. (FC)
11. Kawabata A, Momoi Y, Inoue-Murayama M, Iwasaki T. Canine mdr1 gene mutation in Japan. J Vet Med Sci. 2005;67:1103-7. (FC)
12. Clancy RM, Neufing PJ, Zheng P, O'Mahony M, Nimmerjahn F, Gordon TP, et al. Impaired clearance of apoptotic cardiocytes is linked to anti-SSA/Ro and -SSB/La antibodies in the pathogenesis of congenital heart block. J Clin Invest. 2006;116:2413-22. (FC)
13. Shao DD, Suresh R, Vakil V, Gomer RH, Pilling D. Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation. J Leukoc Biol. 2008;83:1323-33. (FC, ICC)
14. Garnett CT, Talekar G, Mahr JA, Huang W, Zhang Y, Ornelles DA, et al. Latent species C adenoviruses in human tonsil tissues. J Virol. 2009;83:2417-28. (FC)
15. Drewniak A, van Raam BJ, Geissler J, Tool AT, Mook OR, van den Berg TK, et al. Changes in gene expression of granulocytes during in vivo granulocyte colony-stimulating factor/dexamethasone mobilization for transfusion purposes. Blood. 2009;113:5979-98. (FC)
16. Miller LA, Gerriets JE, Tyler NK, Abel K, Schelegle ES, Plopper CG, et al. Ozone and allergen exposure during postnatal development alters the frequency and airway distribution of CD25+ cells in infant rhesus monkeys. Toxicol Appl Pharmacol. 2009;236:39-48. (FC)
17. Montalvão F, Almeida GM, Silva EM, Borges VM, Vasconcellos R, Takiya CM, et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-α secretion and reduce parasite replication in macrophages. Eur J Immunol. 2010;40:417-25. (FC, ICC)
18. Chmielewski M, Hombach AA, Abken H. CD28 cosignalling does not affect the activation threshold in a chimeric antigen receptor-redirected T-cell attack. Gene Ther. 2011;18:62-72. (FC)
19. Fournier P, Aigner M, Schirrmacher V. Targeting of IL-2 and GM-CSF immunocytokines to a tumor vaccine leads to increased anti-tumor activity. Int J Oncol. 2011;38:1719-29. (FC)
20. Guzman R, Valente EG, Pretorius J, Pacheco E, Qi M, Bennett BD, et al. Expression of ORAI1, a plasma membrane resident subunit of the CRAC channel, in rodent and non-rodent species. J Histochem Cytochem. 2014;62:864-78. (FC)
21. Dasso JF, Obiakor H, Bach H, Anderson AO, Mage RG. A morphological and immunohistological study of the human and rabbit appendix for comparison with the avian bursa. Dev Comp Immunol. 2000;24:797-814. (IHC-FS, IHC-PS)
22. Crawford JR, Pilling D, Gomer RH. FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation. J Leukoc Biol. 2012;92:699-711. (ICC)
23. Tosi MF, Zakem H. Surface expression of Fcγ receptor III (CD16) on chemoattractant-stimulated neutrophils is determined by both surface shedding and translocation from intracellular storage compartments. J Clin Invest. 1992;90:462-70. (WB)
24. Rifkin IR, Leadbetter EA, Beaudette BC, Kiani C, Monestier M, Shlomchik MJ, et al. Immune complexes present in the sera of autoimmune mice activate rheumatoid factor B cells. J Immunol. 2000;165:1626-33. (ELISA)
25. Singh N, Johnstone DB, Martin KA, Tempera I, Kaplan MJ, Denny MF. Alterations in nuclear structure promote lupus autoimmunity in a mouse model. Dis Model Mech. 2016;9:885-97. (ELISA)
26. Hande S, Notidis E, Manser T. Bcl-2 obstructs negative selection of autoreactive, hypermutated antibody V regions during memory B cell development. Immunity. 1998;8:189-98. (IHC-FS)
27. Notidis E, Hande S, Manser T. Enforced expression of Bcl-2 selectively perturbs negative selection of dual reactive antibodies. Dev Immunol. 2001;8:223-34. (IHC-FS)
28. Szijártó V, Guachalla LM, Visram ZC, Hartl K, Varga C, Mirkina I, et al. Bactericidal monoclonal antibodies specific to the lipopolysaccharide O antigen from multidrug-resistant Escherichia coli clone ST131-O25b:H4 elicit protection in mice. Antimicrob Agents Chemother. 2015;59:3109-16. (WB)
29. Ohnaga T, Shimada Y, Takata K, Obata T, Okumura T, Nagata T, et al. Capture of esophageal and breast cancer cells with polymeric microfluidic devices for CTC isolation. Mol Clin Oncol. 2016;4:599-602.
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文献和实验相关实验
CD85g/CD85h/CD85i/CD85j/CD85k/CD85l/CD85m分子
CD85g/CD85h/CD85i/CD85j/CD85k/CD85l/CD85m 分子 CD85g 常用单克隆抗体或代号：ILT7 CD85h 常用单克隆抗体或代号：ILT1／LIR7 CD85i 常用单克隆抗体或代号：LIR6 CD85j 常用单克隆抗体或代号：ILT2／LIRl，MIR7 主要表达细胞：NK．M，K，，B，T， 分子质量(kDa)和结构
可能与其他物种发生交叉反应， 除非特异性的经过交叉反应物种的吸附处理。在抗体描述中，经过其他物种的吸附处理的抗体标有“ (min X …Sr Prot) ”字样 。 Anti-IgG (H+L) 这类抗体和 IgG 分子的重链和轻链都可以反应，比如，和 IgG 分子的 Fc ， F(ab ’)2/Fab 部分。 Anti-IgG (H+L) 抗体也可以和其他的免疫球蛋白家族（ IgM ， IgA ，IgD,IgE ）和亚家族反应，因为所有的免疫球蛋白都有相同的轻链（或者 κ 链
，因此将非特异性结合降低到最低。 【Anti-IgG还是Anti-IgG, F（ab’）2 fragment】 在一些如胸腺、脾脏、血液的组织以及造血细胞、淋巴细胞、B细胞等细胞内，通常含有较多的Fc受体，这时候选择二抗时最好选择Anti-IgG, F（ab’）2 fragment这样的特异性二抗，这样就消除了由于Fc部分与IgG的非特异性结合。常规的Anti-IgG （H+L）二抗与IgG的重链和轻链都有结合反应，即与IgG的Fc的F（ab’）2片段都能结合；由于IgG、IgM、IgA都有保守
技术资料暂无技术资料 索取技术资料
山羊F（ab'）2抗鼠Ig G（H+L），人ads-PE
￥4240
