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文献和实验rpm. 13. Decant the supernatant, drain inverted on a paper towel. Dry under vacuum. 14. Resuspend in 50 ml 10:0.1 TE for use in dye primer or dye terminator sequencing chemistry. H. Genomic DNA isolation from blood
Isolation of Genomic DNA from Milk using the Tissue DNA Kit (pdf, 126.00 KB)
实验步骤 1. Isolate DNA from leukocyte in Milk: (up to 50ml milk can be used) 1) If the Milk has been stored at 2-8 EC, a fatty layer of cream should form. Remove the fatty layer of cream from the top of milk by using
kb) 1) Add 0.5 x volume of Buffer P3 and 1 x volume of absolute ethanol (room temperature, 96-100% ) to the DNA solution and vortex to mix well. For example, 100 ul of DNA Solution, add 50 ul of Buffer P3 and 100 ul of absolute ethanol
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