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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
Fast and reliable mini-prep RNA extraction from Neurospora crassa
We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extract more than 50 minipreps at once. Procedures of RNA extraction published so far follow
" standard. 5. Measure each RNA sample. Multiply the results by 10x (dilution factor). DNAse I Digestion 1. Create DNAse I buffer: MgCl2 and KCl should directly treated with 0.1% DEPC, incubated o.n. at room temp
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