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文献和实验Chromatin IP (CHIP assay) This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
Analysis of methylation using bisulphite sequencing
Analysis of methylation using bisulphite sequencing Contributed by Dr. A. Gratchev This method allows precise analysis of methylation in a certain region by converting all nonmethylated cytosines into tymines, while methylated
Methylation analyis using restriction enzyme digestion
Methylation analyis using restriction enzyme digestion Contributed by Dr. A. Gratchev This is a classical method of methylation analysis based on the property of some restriction enzymes to be unable to cut methylated DNA
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