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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
<- 20℃
- 保质期:
>2 years
- 英文名:
Friendbio Science & Technology Co.,Ltd.
- 库存:
200
- 供应商:
福因德科技(武汉)有限公司
- 规格:
20μg
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文献和实验Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
mL PBS 1X 4- Centrifuge (1pulse at 500g) 5- Resuspend in 10 mL PBS 1X (stable for one month at 4 _ C) b) Extract preparation: 1- Transform BL21 or any appropriate bacterial strain with your GST-fused cDNA. Always use freshly transformed cells
GST Fusion Protein Purification from Yeast
GST Fusion Protein Purification from Yeast 5 ml overnight culture of your favorite yeast in your favorite medium. Inoculate 50 ml and grow 30o C shaking O/N until OD600 = 0.8 to 1.2. For SCD cultures
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