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Human ALDOB / Aldolase B Prote

in GST Tag
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  • ¥945.25
  • Frdbio
  • WUHAN,CHINA
  • FrdH4431-909E
  • 2026年02月08日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      <- 20℃

    • 保质期

      >2 years

    • 英文名

      Friendbio Science & Technology Co.,Ltd.

    • 库存

      200

    • 供应商

      福因德科技(武汉)有限公司

    • 规格

      20μg

    分子别名(Synonym):ALDB,ALDO2 种属(Species):Human 表达系统(Expressed Host):E. coli 制剂(Buffer):Lyophilized from sterile PBS, pH 7.5 分子量(Molecule Mass):The recombinant human ALDOB/GST chimera consists of 597 amino acids and has a predicted molecular mass of 66.5 kDa. It migrates as an approxiamtely 60 KDa band in SDS-PAGE under reducing conditions. 内毒素(Endotoxin):<1.0 EU per μg by the LAL method. 生物活性(Activity): 表达区间(Protein Construction):A DNA sequence encoding the human ALDOB (P05062) (Ala 2-Tyr 364) was fused with the GST tag at the N-terminus. 存储条件(Storage): For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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    图标文献和实验
    相关实验
    • Small Scale GST-Tag Purification Under Nature Conditions

        Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook   (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction

    • GST pull down

      mL PBS 1X 4- Centrifuge (1pulse at 500g) 5- Resuspend in 10 mL PBS 1X (stable for one month at 4 _ C) b) Extract preparation: 1- Transform BL21 or any appropriate bacterial strain with your GST-fused cDNA. Always use freshly transformed cells

    • GST Fusion Protein Purification from Yeast

        GST Fusion Protein Purification from Yeast     5 ml overnight culture of your favorite yeast in your favorite medium. Inoculate 50 ml and grow 30o C shaking O/N until OD600 = 0.8 to 1.2.  For SCD cultures

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