Human CD3D & CD3E Heterodimer

分子别名（Synonym）:CD3-DELTA,IMD19,T3D 种属（Species）：Human 表达系统（Expressed Host）：HEK293 Cells 制剂（Buffer）：Lyophilized from sterile PBS, pH 7.4. 分子量（Molecule Mass）：The recombinant heterodimer of human CD3D&CD3E comprises 272 (126+146) amino acids and has a calculated molecular mass of 31.2 (14.4+16.8)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions. 内毒素（Endotoxin）：＜1.0 EU per μg by the LAL method. 生物活性(Activity)：Measured by its binding ability in a functional ELISA. 表达区间（Protein Construction）：A DNA sequence encoding the extracellular domain (Ser2-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Ser2-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. 存储条件（Storage）： For long term storage, the product should be stored at lyophilized state at -20℃ or lower.Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at:4-8℃ for 12 months in lyophilized state;-70℃ for 3 months under sterile conditions after reconstitution. 运输条件： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.