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Store at 2 to 8°C|Protect fluorescent conjugates from light
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文献和实验in DMF (v/v) to deprotect the Fmoc group. Agitate for 2 minutes and drain. Repeat with a 12 minute incubation. 4) Rinse the resin by adding 2 mL of DMF, agitating for 15 seconds, and draining. Repeat 3x. 5) Bromoacetylation
Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells
, disposable 10‐ml pipets (Costar, cat. no. 4488) Castro‐Viejo scissors 37°C, 5% CO 2 incubator
from Maixin Co., Fuzhou.Methods Cell culturing Human hepatoma BEL-7402 cells were grown as monolayers in RPMI 1640 medium supplemented with 8% calf serum (CS), 100IU/mL penicillin and 100mg/mL streptomycin and incubated at 37℃ in the humidified incubator
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