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文献和实验Kingfisher Flex 96 Plant DNA High Pure Protocol
实验试剂 Regents to be provided by user 1. Absolute (96%-100%) ethanol 实验设备 Equipments to be provided by user 1. Centrifuge capable of at least 3,000-5,000 x g 2. Rotor adapter for 96
E.Z.N.A. Cycle-Pure Kit Spin Protocol
. 9. Place HiBind® DNA column into a clean 1.5ml microcentrifuge tube. Add 30-50ul (depending on desired concentration of final product) of Elution Buffer (10mM Tris, pH8.5) or water directly onto the column matrix and centrifuge for 1 min at 13,000
Staining Procedure for Flow Cytometric Detection of Human Cyclins
once by resuspending the pellet in 30-40 ml of Wash buffer. Centrifuge at 200 x g for 10 min and aspirate supernatant. 3. While vortexing, add 10 ml cold 75% ethanol (for D1, use pure cold methanol), drop by drop, to the cell pellet. 4. Incubate at �20°C
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