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- 2026年02月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 保质期：
保质期内
- 英文名：
MKM
- 库存：
充足
- 供应商：
北京美科美生物技术开发有限公司
- CAS号：
来电咨询
- 规格：
10 mg
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文献和实验Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
The method described in the following chapter utilizes a double thymidine block (an inhibitor of DNA synthesis) followed by treatment of cells with nocodazole (a mitotic inhibitor) to obtain large cell populations at distinct phases
Arrest: Make up 2M stock in H 2 O. This is a 10X stock (final 0.2M) Nocodazole Arrest: Make up stock of 1.5 mg/ml in DMSO This is ~100X (see 4). Make culture 1% DMSO prior to adding
inhibitor RO3306. Cells can also be enriched in mitosis by treating with nocodazole and mechanical shake-off. Release of the cells from these blocks enables researchers to follow gene expression and other events through the cell cycle
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