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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
储存于-20 ° C
- 保质期：
1年
- 英文名：
Nocodazole
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
无
- 规格：
10mM (in 1mL DMSO)
Nocodazole，Nocodazole，Nocodazole,Nocodazole
产品货号：APE-A8487-51
产品规格：10mM (in 1mL DMSO)
微管蛋白产生抑制剂，抗肿瘤剂
Ubiquitination/ Proteasome|Autophagy
Nocodazole-Nocodazole储存于-20 ° C
点击：Nocodazole-Nocodazole查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多APExBIO公司特色小分子抑制剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
The method described in the following chapter utilizes a double thymidine block (an inhibitor of DNA synthesis) followed by treatment of cells with nocodazole (a mitotic inhibitor) to obtain large cell populations at distinct phases
Arrest: Make up 2M stock in H 2 O. This is a 10X stock (final 0.2M) Nocodazole Arrest: Make up stock of 1.5 mg/ml in DMSO This is ~100X (see 4). Make culture 1% DMSO prior to adding
inhibitor RO3306. Cells can also be enriched in mitosis by treating with nocodazole and mechanical shake-off. Release of the cells from these blocks enables researchers to follow gene expression and other events through the cell cycle
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