- ￥1049
- Merck-Merck
- 1.16493.0010
- 2026年02月07日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Urease (from jack beans) solution in 50% glycerol, 1000 U/ml for biochemistry EC 3.5.1.5
- 供应商：
Merck
- 库存：
1
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文献和实验ug/ml RNase A; some also add RNase T1 to a final concentration of 10 U/ul) by gentle vortexing, add 0.2 ml of alkaline lysis solution, gently mix, and incubate for 15 minutes at room temperature. 4. Add 0.2 ml of 3M NaOAc, pH 4.8, gently mix
Brdu免疫组织化学染色分析 BrdU incorporation assay
, incubate at RT for 1 hour. Rinse in PBS 3x. Incubate with ABC reagent in PBS containing 0.1% Tween 20 at RT for 1 hour.. (ABC reagent solution: 5ml PBS/0.1% Tween 20 with 1 drop A and 1 drop B, mix well). Rinse with PBS 3x. DAB
Metabolic labeling Immunoprecipitation
/ml Cysteine-HCl,100µg/ml methionine,sterile filtered • Tris-buffered saline (TBS): 20mM Tris,150mM NaCl,pH 7.4 • X1000 Protease inhibitor soln: 500mM PMSF (Sigma P-7626)in EtOH,10mg/ml leupeptin (Sigma L-2023)in water,10mg/ml pepstatin A (P-4265
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