- 询价
- 05-559
- 2026年02月07日
- Western Blotting;Immunocytochemistry
- Mouse
- Bovine;Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunocytochemistry
- 宿主：
Mouse
- 适应物种：
Bovine;Human;Mouse;Rat
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
1
- 规格：
200 μg
Product Images
WB: PC12 cell lysate was probed with anti-Tubulin, Neuronal (1μg/ml)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB, IC | Mouse | Purified | Monoclonal Antibody |
- Western Blotting
- Immunocytochemistry
- Bovine
- Human
- Mouse
- Rat
- TUBB3
- TUBB4
- beta-4
- MC1R
- Neuronal & Glial Markers
- Neurofilament & Neuron Metabolism
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Peptide corresponding to residues 436-450 of neuronal specific βIII tubulin; epitope mapped to EAQGPK 详细描述见链接：http://www.millipore.com/catalogue/item/05-559
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文献和实验1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
General Protocol to Prepare Neuronal Cultures
Substrate Preparation 1. Prepare culture plate by coating with poly-D-lysine (0.15 ml/cm2, 50 μg/ml, 135 kD) 1-20 hr., and rinse one time with 18 Mohm deionized water, and let dry. Preparation of Isolated Neurons 1. Store tissue at 4°C
Dual- and Triple-Color Fluorospot
of 35% ethanol for 1 min at room temperature and wash the plate five times with 200 μl of sterile water per well. b. Mix coating mAbs for IFN-γ and IL-2 in the same tube in sterile PBS, pH 7.4, at a final concentration of 15 μg/ml each. Add
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