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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
ELISA;Western Blotting
- 宿主:
Rabbit
- 库存:
大量
- 抗原来源:
0
- 适应物种:
Rat
- 是否单克隆:
2
- 规格:
50 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
- ELISA
- Western Blotting
ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide per well.
Optimal working dilutions must be determined by the end user.
- SLC8A1
- NCX1
- DKFZp779F0871
- +Ca
- CNC
- Na
- MGC119581
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
A 23 amino acid peptide sequence within the cytoplasmic domain 3 rat NCX1 (Low et al. 1993; Furman et al. 1993; Lee et al. 1994; Nicoll et al. 1996; Kumoro et al. 1992; Blaustein & Lederer 1999). 详细描述见链接:http://www.millipore.com/catalogue/item/AB3516P
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文献和实验for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
Stewart Laboratory Standard Operating Procedure
alone stain is desired, set aside a fraction of cells until step 10). Resuspend cell pellet in anti-BrdU antibody (50 μL total solution diluted with wash buffer). Incubate for 20 minutes at 4°C. Use BrdU-FITC: Boeringher #1202693 1:100 dilution (skip to step
Stewart Laboratory Standard Operating Procedure
in anti-BrdU antibody (50 μL total solution diluted with wash buffer). Incubate for 20 minutes at 4°C. Use BrdU-FITC: Boeringher #1202693 1:100 dilution (skip to step 9) Or anti-BrdU: Boeringher #1170376 1:10 dilution Wash cells
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