5-hmC Enrichment Kit

Human DC Enrichment Kit

once with Buffer 1 by centrifugation at 400 x g for 8 minutes at 2–8°C. 8)        Wash MNC twice with Buffer 1 by centrifugation at 225 x g for 8 minutes at 2–8°C and resuspend the MNC at 1 x 108 MNC per ml in Buffer 1. 3.        Enrichment of DCs

Ribominus Transcriptome Isolation Kit (Yeast and Bacteria) - for efficient transcriptome enrichment by depleting large

through.  4) Repeat the wash step once.  5) Discard the tube and place the column into a clean Wash Tube, supplied with the kit.  6) Centrifuge the column at ≥12,000 × g for 2–3 minutes at room temperature to remove any residual Wash Buffer

Active Motifs ChIP-IT Kit

fixation, 2) immunoprecipitation of specific chromatin fragments using antibody against the protein of interest and 3) analysis of immunoprecipitated fractions to determine if target DNA is bound by the protein of interest (evidenced by enrichment of DNA