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文献和实验Ultraviolet Crosslinking of DNA-Protein Complexes via SAzidoadenine
In biological systems photoreactive derivatives have been widely applied to study specific interactions of receptor molecules with their ligands by photoaffinity labeling (1 –3 ).
Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
In biological systems, photoreactive derivatives have been widely applied to study specific interactions of receptor molecules with their ligands by photoaffinity labeling (1 –3 ). While the receptors are generally proteins (e.g., enzymes
Ultraviolet Crosslinking of DNA Protein Complexes via 8-Azidoadenine
. After incorporation of this dATP analog by nick translation into DNA containing the tet operator sequence the investigation of the interactions between tet operator DNA and Tet repressor becomes possible. Photocrosslinking of protein to DNA was demonstrated
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